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Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8887R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1127B

Product Summary for Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Antibody

Immunogen

HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
Met1-His245
Accession # Q14954

Specificity

Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844P) is recommended.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Antibody

Detection of KIR2DS1/CD158h antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8887R) and either (A) Mouse Anti-Human KIR2DL1/KIR2DS5 PE-conjugated Monoclonal Antibody (Catalog # FAB1844P) or (B) Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts. Over amino acids (aa) 1-245, human KIR2DS1 shares 97% aa sequence identity with human KIR2DL1.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Alternate Names

CD158h, EB6ActI

Entrez Gene IDs

3806 (Human)

Gene Symbol

KIR2DS1

UniProt

Additional KIR2DS1/CD158h Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human KIR2DS1/CD158h Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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