Human/Mouse/Rat Actin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4000
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot, Western Blot Control
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Summary for Human/Mouse/Rat Actin Antibody
Specificity
Detects human, mouse, and rat Actin in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Actin Antibody
Detection of Human/Mouse/Rat Actin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Actin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Actin in HeLa Human Cell Line.
Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Actin in HEK293 Human Cell Line.
Actin was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Actin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Actin
The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.
Alternate Names
ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
Additional Actin Products
Product Documents for Human/Mouse/Rat Actin Antibody
Product Specific Notices for Human/Mouse/Rat Actin Antibody
For research use only
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