Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4849
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 731502
Product Specifications
Immunogen
E. coli-derived recombinant human Crystallin/CRYAB
Met1-Lys175
Accession # P02511
Met1-Lys175
Accession # P02511
Specificity
Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Detection of Human, Mouse, and Rat AlphaB Crystallin/CRYAB by Western Blot.
Western blot shows lysates of rat heart tissue, mouse heart tissue, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human AlphaB Crystallin/CRYAB by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 28 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Application
Recommended Usage
Simple Western
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Western Blot
0.1 µg/mL
Sample: Rat heart tissue, mouse heart tissue, and human heart tissue
Sample: Rat heart tissue, mouse heart tissue, and human heart tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AlphaB Crystallin/CRYAB
Alternate Names
CRYA2, CRYAB, CTPP2, HSPB5
Gene Symbol
CRYAB
UniProt
Additional AlphaB Crystallin/CRYAB Products
Product Documents for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
Product Specific Notices for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody
For research use only
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