Human/Primate IL-6 Antibody Best Seller

R&D Systems, part of Bio-Techne | Catalog # MAB206

R&D Systems, part of Bio-Techne
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MAB206-100
MAB206-500
MAB206-SP
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Neutralization, Western Blot

Cited:

Affinity Chromatography, Antibody Array Development, Array Development, Block/Neutralize, Electrochemiluminescent Assay, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Microfluidic Assay, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6708

View all IL-6 Antibodies »

Product Specifications

Specificity

Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑6, rhOSM, rhLIF, rhIL‑11, rhgp130, or rhCNTF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Primate IL-6 Antibody

Detection of Recombinant Human IL-6 antibody by Western Blot.

Detection of Recombinant Human IL‑6 by Western Blot.

Western blot shows 25 ng of Recombinant Human IL-6 (Catalog # 206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
IL-6 antibody in Human Skin by Immunohistochemistry (IHC-Fr).

IL‑6 in Human Skin.

IL-6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody.

Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody.

Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206). The ND50 is typically 8.00 - 80.0 ng/mL.

Applications for Human/Primate IL-6 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of hyperplastic human skin

Western Blot

1 µg/mL
Sample: Recombinant Human IL-6 (Catalog # 206-IL)

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 8.00 - 80.0 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Human/Primate IL-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate IL-6 Biotinylated Antibody (Catalog # BAF206)
  • Standard: Recombinant Human IL-6 Protein (Catalog # 206-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 16 reviews rated 4.5 using MAB206 in the following applications:

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Human/Primate IL-6 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Primate IL-6 Antibody

For research use only

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