IL-12/IL-35 p35 Antibody (27537) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1570A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 27537
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for IL-12/IL-35 p35 Antibody (27537) [Allophycocyanin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-12/IL-35 p35
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Long Name
Interleukin 12 p35
Alternate Names
IL-12A, IL35p35
Gene Symbol
IL12A
Additional IL-12/IL-35 p35 Products
Product Documents for IL-12/IL-35 p35 Antibody (27537) [Allophycocyanin]
Product Specific Notices for IL-12/IL-35 p35 Antibody (27537) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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