IRE1 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2324
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
This IRE1 alpha antibody was raised against a synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Localization
Endoplasmic reticulum
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
NB100-2324 is useful for the detection of endogenous total IRE1 Alpha protein. For detecting phospho-IRE1 Alpha (Ser-724), we suggest NB100-2323 and it is recommended to normalize phospho-IRE1 alpha band intensity/immunoreactivity with total-IRE1 alpha using NB100-2324 or NB110-59971.
Scientific Data Images for IRE1 alpha Antibody
Immunohistochemistry: IRE1 alpha Antibody [NB100-2324] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license.
Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.
Applications for IRE1 alpha Antibody
Application
Recommended Usage
Immunocytochemistry/Immunofluorescence
1:100 - 1:250
Immunohistochemistry
1:250 - 1:500
Immunohistochemistry-Paraffin
1:250 - 1:500
Knockout Validated
reported in scientific literature (PMID 31159306)
Western Blot
1:1000 - 1:2000
Application Notes
NB100-2324 is useful for the detection of endogenous total IRE1 Alpha protein. For detecting phospho-IRE1 Alpha (Ser-724), we suggest NB100-2323 and it is recommended to normalize phospho-IRE1 alpha band intensity/immunoreactivity with total-IRE1 alpha using NB100-2324 or NB110-59971. In Western blot a band at ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-2324 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IRE1 alpha
Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.
IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.
References
1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3
2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0
3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y., . . . Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353
Long Name
Serine/threonine-protein kinase/endoribonuclease IRE1
Alternate Names
ERN1, hIRE1p, IRE1, Ire1-alpha
Gene Symbol
ERN1
Additional IRE1 alpha Products
Product Documents for IRE1 alpha Antibody
Product Specific Notices for IRE1 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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