Islet-1 Antibody (1G9Q8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15315
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G9Q8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Islet-1 Antibody (1G9Q8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Islet-1 (P61371). YAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQ
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Islet-1 Antibody (1G9Q8)
Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Immunohistochemistry of paraffin-embedded mouse fetal Heart using Islet-1 Rabbit mAb (NBP3-15315) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Applications for Islet-1 Antibody (1G9Q8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Islet-1
Alternate Names
ISL1, Islet1
Gene Symbol
ISL1
Additional Islet-1 Products
Product Documents for Islet-1 Antibody (1G9Q8)
Product Specific Notices for Islet-1 Antibody (1G9Q8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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