Islet-1 Antibody (2E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00003670-M05
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Functional, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Islet-1 Antibody (2E7)
Immunogen
ISL1 (NP_002193, 63 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
Localization
Nuclear, occasionally cytoplasmic
Specificity
ISL1 - ISL1 transcription factor, LIM/homeodomain, (islet-1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Islet-1 Antibody (2E7)
Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).
Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml
Applications for Islet-1 Antibody (2E7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IP usage reported in scientific literature (PMID: 25070240). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 26635867).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Islet-1
Alternate Names
ISL1, Islet1
Entrez Gene IDs
3670 (Human)
Gene Symbol
ISL1
OMIM
600366 (Human)
UniProt
Additional Islet-1 Products
Product Documents for Islet-1 Antibody (2E7)
Product Specific Notices for Islet-1 Antibody (2E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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