MAP2 Antibody

Immunofluorescent Analysis of MAP2 in Treated Rat Hippocampi

PEA decreases astrocyte activation in organotypic cultures of rat hippocampi and rescues neuronal CA3 damage caused by Abeta challenge. Abeta-challenged (1 ug/ml) slices of rat hippocampi were treated for 24 hours with PEA (0.1 uM) in the presence of the selective PPAR? antagonist (GW9662, 9 nM) or the selective PPARalpha antagonist (MK886, 3 uM). Representative photomicrographs of the CA3 region showing the results of immunofluorescence experiments aimed at investigating the effect of treatments on astrocyte activation and neuronal loss, as determined by immunostaining for GFAP (green) and MAP2 (red) alone or merged, respectively. Nuclei were stained with Hoechst (blue). Scale bar: 10 um. Arrows in the photomicrographs indicate astrocyte infiltration events and apoptotic condensation in the nuclei of adjacent neurons.

Immunocytochemistry/Immunofluorescence Staining of MAP2 in Human iPS Derived Neurons

Human iPS derived neurons labeled with MAP2 (in green) at 1:5000 dilution. Image from verified customer review.

Immunohistochemical Analysis of MAP2 in Frozen Retina

MAP2 in the retina detected by NB300-213 antibody (magenta). DAPI (blue). Image from verified customer review.

Immunocytochemistry/Immunofluorescence Staining of MAP2 in Human DA Neurons

Human DA neurons 28 DIV stained with MAP2 antibody (blue). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - PEA inhibits astroglial proliferation and reduces neuronal loss in mixed neuroglia co-cultures exposed to Abeta. Abeta-challenged (1 ug/ml) astrocyte/neuron mixed cultures were treated with PEA (0.1 uM) in the presence of the selective PPAR-gamma antagonist (GW9662, 9 nM) or the selective PPARalpha antagonist (MK886, 3 uM). After 24 hours of treatment, cells were processed for analyses. Immunofluorescence photomicrographs showing the effect of the treatments on astrocyte proliferation and neuronal loss, as determined by immunostaining for GFAP (green) and MAP2 (red), respectively. Arrows indicate chromatin condensation in nuclei stained with Hoechst dye (blue) as markers of apoptotic events. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-49), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Oxytocin receptors (Oxtr) are expressed in mouse hippocampus. Confocal images of Oxtr-expressing neurons in the CA1 subfields of the medial and caudal hippocampus from OxtrVn/+ sections, triple stained with antibodies directed against NeuN (red), MAP2 (magenta), and Venus (green). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/22466), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Differential and neuron-specific Oxtr expression in mouse hippocampus. Immunohistochemical mapping of Venus expression in coronal sections of the rostral, medial, and caudal hippocampus from three weeks old OxtrVn/+ mice triple-labeled with antibodies against NeuN (red), Venus (green), and Map2 (magenta). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/22466), licensed under a CC-BY license.