Mouse alpha1-Acid Glycoprotein Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5934

R&D Systems, part of Bio-Techne
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AF5934
AF5934-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all alpha 1-Acid Glycoprotein Antibodies »

Product Summary for Mouse alpha1-Acid Glycoprotein Antibody

Immunogen

Mouse plasma-derived alpha1‑Acid Glycoprotein
Accession # P02763

Specificity

Detects mouse alpha1‑Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with human alpha1‑Acid Glycoprotein is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse alpha1-Acid Glycoprotein Antibody

Detection of Mouse a1-Acid Glycoprotein antibody by Western Blot.

Detection of Mouse alpha1-Acid Glycoprotein by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a1-Acid Glycoprotein at approximately 43kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse alpha1-Acid Glycoprotein Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Acid Glycoprotein

alpha1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.

Alternate Names

AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid

Entrez Gene IDs

5004 (Human); 18405 (Mouse); 24614 (Rat)

Gene Symbol

ORM1

UniProt

P02763

Additional alpha 1-Acid Glycoprotein Products

Product Documents for Mouse alpha1-Acid Glycoprotein Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse alpha1-Acid Glycoprotein Antibody

For research use only

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