Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 983802

View all Fibroblast Activation Protein alpha/FAP Antibodies »

Product Specifications

Specificity

Detects mouse Fibroblast Activation Protein  alpha/FAP in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Fibroblast Activation Protein  alpha/FAP Antibody

Detection of Fibroblast Activation Protein alpha/FAP antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Fibroblast Activation Protein alpha/FAP Monoclonal Antibody (Catalog # MAB9727, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Fibroblast Activation Protein a/FAP antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line.

Fibroblast Activation Protein a/FAP was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB9727) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Fibroblast Activation Protein  alpha/FAP Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Fibroblast Activation Protein  alpha/FAP Monoclonal Antibody (Catalog # MAB97271).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).

References

  1. Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
  2. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  3. Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
  4. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  5. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
  7. Bauer, S. et al. (2006) Arthritis Res. 8:R171.
  8. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
  9. Keane, F.M. et al. (2011) FEBS J. 278:1316.
  10. Ghersi, G. et al. (2006) Cancer Res. 66:4652.
  11. Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
  12. Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
  13. Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
  14. Kraman, M. et al. (2010) Science 330:827.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Gene Symbol

FAP

UniProt

P97321 (Mouse)

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Mouse Fibroblast Activation Protein  alpha/FAP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Fibroblast Activation Protein  alpha/FAP Antibody

For research use only

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