Integrin a2b/CD41 was detected in immersion fixed mouse bone marrow cells using Rat Anti-Mouse Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB41181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Integrin alpha2b/CD41 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse platelets

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse platelets and immersion fixed mouse bone marrow cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2b/CD41

Integrin alpha 2b, (ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. ITGA2b associates with integrin beta3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. ITGA2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)

Gene Symbol

ITGA2B

UniProt

NP_034705

Additional Integrin alpha 2b/CD41 Products

Product Documents for Mouse Integrin alpha2b/CD41 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Integrin alpha2b/CD41 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Integrin alpha2b/CD41 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB41181

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Summary for Mouse Integrin alpha2b/CD41 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Integrin alpha2b/CD41 Antibody

Integrin alpha2b/CD41 in Mouse Bone Marrow Cells.