Mouse Siglec-3/CD33 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2220
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 925763
Product Specifications
Specificity
Detects mouse Siglec‑3/CD33 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Siglec-3/CD33 Antibody
Detection of Mouse Siglec‑3/CD33 by Western Blot.
Western blot shows lysates of EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Siglec-3/CD33 Monoclonal Antibody (Catalog # MAB2220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Siglec-3/CD33 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Siglec-3/CD33 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: EL‑4 mouse lymphoblast cell line
Sample: EL‑4 mouse lymphoblast cell line
Formulation, Preparation, and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
UniProt
Additional Siglec-3/CD33 Products
Product Documents for Mouse Siglec-3/CD33 Antibody
Product Specific Notices for Mouse Siglec-3/CD33 Antibody
For research use only
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