NF-M Antibody (1B6B12) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61659
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1B6B12
Format
BSA Free
Concentration
1 mg/ml
Product Summary for NF-M Antibody (1B6B12) - BSA Free
Immunogen
Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
102.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NF-M Antibody (1B6B12) - BSA Free
Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)
Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).
Applications for NF-M Antibody (1B6B12) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF-M
Long Name
Neurofilament Protein, Medium Chain
Alternate Names
NEF3, NEFM, NFM
Gene Symbol
NEFM
Additional NF-M Products
Product Documents for NF-M Antibody (1B6B12) - BSA Free
Product Specific Notices for NF-M Antibody (1B6B12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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