Podocalyxin Like Antibody (2A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45169
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Rabbit
Applications
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # 2A4
Concentration
0.2 mg/ml
Product Summary for Podocalyxin Like Antibody (2A4)
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)
Localization
Cell surface and Cytoplasmic
Specificity
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47750)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Scientific Data Images for Podocalyxin Like Antibody (2A4)
Western Blot: Podocalyxin Like Antibody (2A4) [NBP2-45169] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (2A4).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) [NBP2-45169] - Human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) [NBP2-45169] - Human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).
Applications for Podocalyxin Like Antibody (2A4)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/Immunofluorescence
1-2 ug/ml
Immunofluorescence
1:25 - 1:50
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Podocalyxin
Additional Podocalyxin Products
Product Documents for Podocalyxin Like Antibody (2A4)
Product Specific Notices for Podocalyxin Like Antibody (2A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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