SARS-CoV-2 ORF3a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10706
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1035921
Product Summary for SARS-CoV-2 ORF3a Antibody
Immunogen
SARS-CoV-2 ORF3a peptide
Specificity
Detects SARS-CoV-2 ORF3a in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SARS-CoV-2 ORF3a Antibody
Detection of SARS-CoV-2 ORF3a by Western Blot.
Western blot shows recombinant SARS-CoV-2 ORF3a peptide. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ORF3a at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
ORF3a in HEK293 Human Cell line Transfected with SARS-CoV-2 ORF3a.
ORF3a was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Mouse Anti-SAR-CoV-2 ORF3a Monoclonal Antibody (Catalog # MAB10706) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of SARS-CoV-2 ORF3a by Simple WesternTM.
Simple Western lane view shows recombinant SARS-CoV-2 ORF3a, loaded at 0.2 mg/mL. A specific band was detected for ORF3a at approximately 34 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for SARS-CoV-2 ORF3a Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a
Simple Western
20 µg/mL
Sample: Recombinant SARS-CoV-2 ORF3a
Sample: Recombinant SARS-CoV-2 ORF3a
Western Blot
1 µg/mL
Sample: Recombinant SARS-CoV-2 ORF3a
Sample: Recombinant SARS-CoV-2 ORF3a
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ORF3a
Additional ORF3a Products
Product Documents for SARS-CoV-2 ORF3a Antibody
Product Specific Notices for SARS-CoV-2 ORF3a Antibody
For research use only
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