SARS-CoV-2 Spike S2 Subunit Antibody

R&D Systems, part of Bio-Techne | Catalog # AF10774

R&D Systems, part of Bio-Techne
Catalog #
Availability
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AF10774-100
AF10774-SP
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Spike S2 Subunit Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived SARS-CoV-2 Spike S2 Subunit
Ser686-Lys1211
Accession # YP_009724390.1

Specificity

Detects SARS-CoV-2 Spike S2 Subunit  in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Spike S2 Subunit Antibody

Detection of SARS-CoV-2 Spike S2 Subunit by Western Blot.

Western blot shows recombinant SARS-CoV-2 Spike S2. PVDF membrane was probed with 1 µg/mL of Goat Anti-SARS-CoV-2 Spike S2 Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Spike S2 Subunit at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Spike S2 Subunit in HEK293 human embryonic kidney cell line transfected with SARS-CoV-2.

Spike S2 Subunit was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Goat Anti-SARS-CoV-2 Spike S2 Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Spike S2 Subunit in SARS-CoV-2 Infected Human Lung.

Spike S2 Subunit was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung using Goat Anti-SARS-CoV-2 Spike S2 Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10774) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to immunoreactive profiles scattered throughout the tissue. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for SARS-CoV-2 Spike S2 Subunit Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung

Western Blot

1 µg/mL
Sample: Recombinant SARS-CoV-2 Spike S2

Reviewed Applications

Read 1 review rated 4 using AF10774 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S2 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (2-4). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV2, similar to SARS-CoV-1, through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (5, 6). The S2 subunit of SARS-CoV-2 shares 90% and 41% amino acid sequence identity with the S2 subunit of SARS-CoV-1 and MERS, respectively. It has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (7, 8).

References

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Bosch, B.J. et al. (2003). J. Virol. 77:8801.
  3. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:5871.
  4. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  5. Li, W. et al. (2003) Nature 426:450.
  6. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  7. Wang, X. et al. (2020) https://doi.org/10.1038/s41423-020-0424-9.
  8. Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v.

Long Name

Spike Protein, S2 Subunit

Alternate Names

COVID-19, S2 protein, SARS-CoV-2, SARS-CoV-2 Spike, Spike glycoprotein Subunit 2, spike protein, Spike protein S2, structural protein, surface glycoprotein

UniProt

YP_009724390.1

Additional Spike S2 Subunit Products

Product Documents for SARS-CoV-2 Spike S2 Subunit Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Spike S2 Subunit Antibody

For research use only

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