SDHB Antibody (CL0346)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30677
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL0346
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SDHB Antibody (CL0346)
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATY
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Marker
Mitochondria Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SDHB Antibody (CL0346)
Western Blot: SDHB Antibody (CL0346) [NBP2-30677] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4
Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677] - Staining of human testis shows moderate cytoplasmic immunoreactivity in the seminiferous tubules.
Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677] - Staining of human liver shows moderate cytoplasmic positivity in the hepatocytes.
Applications for SDHB Antibody (CL0346)
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SDHB
Alternate Names
EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Symbol
SDHB
UniProt
Additional SDHB Products
Product Documents for SDHB Antibody (CL0346)
Product Specific Notices for SDHB Antibody (CL0346)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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