SOX10 Antibody (SOX10/1074)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59622
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SOX10/1074
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary) (Uniprot: P56693 )
Localization
Nuclear
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This monoclonal antibody is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Human Chromosome Location: 22q13.1
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59623)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59623)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for SOX10 Antibody (SOX10/1074)
Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29
Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Western blot analysis of COLO-38 cell lysate using SOX10 antibody (SOX10/1074).
Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).
Applications for SOX10 Antibody (SOX10/1074)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Protein Array
1:100-1:2000
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined. SOX10 Antibody (SOX10/1074) validated for Simple Western from a verified customer review.
Optimal dilution for a specific application should be determined. SOX10 Antibody (SOX10/1074) validated for Simple Western from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using NBP2-59622 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SOX10
Additional SOX10 Products
Product Documents for SOX10 Antibody (SOX10/1074)
Product Specific Notices for SOX10 Antibody (SOX10/1074)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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