TMEM119 Antibody (CL8714)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76985
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL8714
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence: GDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPS
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TMEM119 Antibody (CL8714)
Western Blot: TMEM119 Antibody (CL8714) [NBP2-76985] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/Immunofluorescence: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of SH-SY5Y cells using the Anti-TMEM119 monoclonal antibody, showing specific staining to the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Applications for TMEM119 Antibody (CL8714)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/mL
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/mL
Application Notes
For IHC, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 5 using NBP2-76985 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TMEM119
ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334).
Long Name
Transmembrane Protein 119
Alternate Names
OBIF
Gene Symbol
TMEM119
Additional TMEM119 Products
Product Documents for TMEM119 Antibody (CL8714)
Product Specific Notices for TMEM119 Antibody (CL8714)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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