Tyrosine Hydroxylase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05555

Novus Biologicals, part of Bio-Techne
Catalog #
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Size / Price
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NBP3-05555-50ul
NBP3-05555-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tyrosine Hydroxylase Antibodies »

Product Specifications

Immunogen

Full length human Tyrosine Hydroxylase as expressed in and purified from E. coli.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tyrosine Hydroxylase Antibody

Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555]

Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555]

Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555]

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555]

Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555] - Immunofluorescent analysis of rat brain section stained with Tyrosine Hydroxylase Antibody at a dilution of 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Tyrosine Hydroxylase Antibody stains the dopaminergic TH-positive neurons and their processes of the substantia nigra.

Applications for Tyrosine Hydroxylase Antibody

Application
Recommended Usage

Immunocytochemistry/Immunofluorescence

1:5000

Immunohistochemistry

1:5000

Western Blot

1:50000

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as an aliquot of IgY preparation

Preservative

5mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Tyrosine Hydroxylase

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1) . Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Alternate Names

TH, TYH

Gene Symbol

TH

Additional Tyrosine Hydroxylase Products

Product Documents for Tyrosine Hydroxylase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tyrosine Hydroxylase Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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