Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 7A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VEGFR3/Flt-4 Antibodies »

Product Summary for VEGFR3/Flt-4 Antibody (7A3) - Azide and BSA Free

Immunogen

Purified, insect cell-derived recombinant mouse soluble FLT-4 (120 kDa)

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for VEGFR3/Flt-4 Antibody (7A3) - Azide and BSA Free

Western Blot: VEGFR3/Flt-4 Antibody (7A3) [NBP1-18653] - Analysis with VEGFR-3/FLT-4 expressing PAEC endothelial cells.
Immunocytochemistry/Immunofluorescence: VEGFR3/Flt-4 Antibody (7A3) [NBP1-18653] - Staining (green) of VEGFR-3/FLT-4 in primary mouse endothelial cells with anti-mouse VEGFR-3/FLT-4 #7A3 (10ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:400.
Immunocytochemistry/Immunofluorescence: VEGFR3/Flt-4 Antibody (7A3) [NBP1-18653] - Immunofluorescence staining of VEGFR-3/FLT4 in mouse primary endothelial cells (SnoMec) with monoclonal rat anti-mouse VEGFR-3/FLT4 #7A3 (upper panel). Control with goat anti-rat ALEXA Flour 488 [Dianova] (lower panel).

Applications for VEGFR3/Flt-4 Antibody (7A3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR3/Flt-4

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Entrez Gene IDs

2324 (Human)

Gene Symbol

FLT4

Additional VEGFR3/Flt-4 Products

Product Documents for VEGFR3/Flt-4 Antibody (7A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VEGFR3/Flt-4 Antibody (7A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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