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Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0017

Novus Biologicals, part of Bio-Techne
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KA0017

Key Product Details

Sample Type & Volume Required Per Well

Breast Milk, Cerebrospinal Fluid, Plasma (Citrate, EDTA, Heparin), Serum, Urine (100 uL)

Sensitivity

0.47 ng/mL (serum, plasma), 0.156 ng/mL (urine, CSF)

Assay Range

1 to 100 ng/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. ADIPOQ (Human) High Sensitivity ELISA Kit is a sandwich enzymelinked immunosorbent assay for the quantitative measurement of human adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.90%

Inter-Assay Precision (Precision between assays) CV% < 6.00%

Recovery for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Recovery

90.50%

Scientific Data Images for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

ELISA: Adiponectin/Acrp30 ELISA Kit [HRP] [KA0017] - Typical Standard Curve for ADIPOQ (Human) ELISA Kit

Kit Contents for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

  • Antibody Coated Microtiter Strips
  • Conjugate Solution
  • Dilution Buffer Conc. (10x)
  • Quality Control HIGH
  • Quality Control LOW
  • Set of Standards
  • Stop Solution
  • Substrate Solution
  • Wash Solution Conc. (10x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

Additional Adiponectin/Acrp30 Products

Product Documents for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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