Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83739

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 uL)

Sensitivity

0.01 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Adiponectin/Acrp30 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Adiponectin positive sample. There was no cross reactivity or interference detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.2%

Inter-Assay Precision (Precision between assays) CV% < 3.1%

Recovery for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Recovery

For recovery data see the manual, table 5.

Scientific Data Images for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

ELISA: Adiponectin/Acrp30 ELISA Kit [NBP1-83739] - Standard curve

Kit Contents for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Adiponectin monoclonal antibody
  • Human Adiponectin Standard lyophilized, 30 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adiponectin/Acrp30

Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. Adiponectin automatically self-associates into larger structures. Adiponectin binds to a number of receptors. So far, two receptors have been identified, with homology to G protein-coupled receptors. Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions, and can have additive effects. Adiponectin affects glucose flux (gluconeogenesis, glucose uptake), lipid catabolism (beta-oxidation, triglyceride clearance), endothelial dysfunction, insulin sensitivity and weight loss. Administration of Adiponectin remains a promising area of research for clinical therapy in diseases such as obesity, type 2 diabetes and fatty liver disease.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

Additional Adiponectin/Acrp30 Products

Product Documents for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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