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Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1023

Novus Biologicals, part of Bio-Techne
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KA1023

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, CSF, Milk, Plasma, Saliva, Serum, Urine (50 uL)

Sensitivity

1.7 ng/mL

Assay Range

3.125 to 200 ng/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

ORM (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human ORM.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.30%

Inter-Assay Precision (Precision between assays) CV% < 7.00%

Recovery for Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

Recovery

97.50%

Scientific Data Images for Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

ELISA: alpha 1-Acid Glycoprotein ELISA Kit [HRP] [KA1023] - Standard curve

Kit Contents for Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

  • Biotinylated AGP Antibody (30x): A 30-fold biotinylated polyclonal antibody against human AGP (300 µl).
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine (8 ml).
  • Human AGP Microplate: A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human AGP.
  • Human AGP Standard: Human AGP in a buffered protein base (200 ng, lyophilized).
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base (30 ml).
  • Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 ml).
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (90 µl).
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant (30 ml).

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha 1-Acid Glycoprotein

Alpha 1 Acid Glycoprotein encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression.

Alternate Names

AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid

Gene Symbol

ORM1

Additional alpha 1-Acid Glycoprotein Products

Product Documents for Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human alpha 1-Acid Glycoprotein ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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