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Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0550

Novus Biologicals, part of Bio-Techne
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KA0550

Key Product Details

Sample Type & Volume Required Per Well

Body Fluid, Cell Culture Supernatant, Serum, Tissue Lysate (100 uL)

Sensitivity

1 pg/mL

Assay Range

15.6 to 1000 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Quality control test: Standard curve. Tnfsf8 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Tnfsf8.

Kit Contents for Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

  • ABC diluent buffer: 12ml.
  • Antibody diluent buffer: 12ml.
  • Avidin-Biotin-Peroxidase Complex (ABC) : 130µl, dilution 1:100.
  • Biotinylated anti- mouse CD30L antibody : 130µl, dilution 1:100.
  • Lyophilized recombinant mouse CD30L standard: 10ng/tube×2.
  • One 96-well plate precoated with anti- mouse CD30L antibody.
  • Sample diluent buffer: 30 ml
  • TMB color developing agent: 10ml.
  • TMB stop solution: 10ml.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD30 Ligand/TNFSF8

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Alternate Names

CD153, CD30L, TNFSF8

Gene Symbol

TNFSF8

Additional CD30 Ligand/TNFSF8 Products

Product Documents for Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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