Human G-CSF DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY214
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
31.2-2000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human G-CSF DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human G-CSF DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human G-CSF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human G-CSF DuoSet ELISA
Human G-CSF ELISA Standard Curve
Kit Contents for Human G-CSF DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
Normal Goat Serum: (Catalog # DY005)
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Normal Goat Serum: (Catalog # DY005)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
Human G-CSF
receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to the
hematopoietin receptor superfamily. The mature protein consists of a 603 aa extracellular
domain (ECD), a 23 aa transmembrane segment, and a 186 aa cytoplasmic domain.
The ECD contains an N-terminal Ig-like domain, a cytokine receptor homology
domain, and three fibronectin type III domains. Alternate splicing of human
G-CSF R generates additional isoforms including a potentially soluble form of
the receptor. The ECDs of mouse and human G-CSF R share 63% aa sequence identity.
G-CSF R forms a complex with the ligand in a 2:2 ratio. It is expressed on
monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors,
trophoblasts and placenta, endothelial cells, and various tumor cell types.
G-CSF is an
important regulator for granulopoiesis in vivo, and mutations in G-CSF R are associated
with congenital neutropenia. G-CSF can support the growth of multilineage
hematopoietic progenitor cells and mobilize them from the bone marrow into the bloodstream.
G-CSF enhances the functional capacity of mature neutrophils and supports their
survival by limiting the rate of apoptosis. G-CSF also enhances M-CSF
induced monocytopoiesis from hematopoietic progenitor cells and stimulates the proliferation
of peripheral Th2-inducing dendritic cells (30, 31). It promotes the
development of T cell immune tolerance as well as tissue recovery following
myocardial infarction and cerebral ischemia.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
Additional G-CSF Products
Product Documents for Human G-CSF DuoSet ELISA
Product Specific Notices for Human G-CSF DuoSet ELISA
For research use only
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