Human IL-6 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89869

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

0.92 pg/mL

Assay Range

1.56 - 100 pg/mL

View all IL-6 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6 positive serum. There was no crossreactivity detected

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.4%

Inter-Assay Precision (Precision between assays) CV% < 5.2%

Recovery for Human IL-6 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 78% to 105% with an overall mean recovery of 88%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human IL-6 ELISA Kit (Colorimetric)

ELISA: IL-6 ELISA Kit [NBP1-89869] - Standard curve

Kit Contents for Human IL-6 ELISA Kit (Colorimetric)

  • * reagent contains ethyl alcohol
  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
  • Amplification Diluent Concentrate (2x)
  • Amplification Reagent I*
  • Amplification Reagent II
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-6 monoclonal antibody
  • Control high, lyophilized
  • Control low, lyophilized
  • Human IL-6 Standard lyophilized, 200 pg/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-6

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide (LPS) or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in: - the induction of B-cell differentiation - the induction of acute phase proteins in liver cells - growth promotion of myeloma/plasmacytoma/hybridoma cells - induction of IL-2 and IL-2 receptor expression - proliferation and differentiation of T cells - inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages - enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor - induction of mesangial cell growth - induction of neural differentiation of PC 12 cells - induction of keratinocyte growth The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Human IL-6 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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