Human MMP-9 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82422

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (10 uL (prediluted 1 : 25) )

Sensitivity

0.05 ng/mL

Assay Range

0.23 - 15.0 ng/mL

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Product Summary for Human MMP-9 ELISA Kit (Colorimetric)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MMP-9 positive sample. There was no cross reactivity or interference detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.3%

Inter-Assay Precision (Precision between assays) CV% < 10.2%

Recovery for Human MMP-9 ELISA Kit (Colorimetric)

Recovery

Recoveries were determined in 2 independent experiments with 4 replicates each. The overall mean recovery was 103.1%.

Scientific Data Images for Human MMP-9 ELISA Kit (Colorimetric)

ELISA: MMP-9 ELISA Kit [Biotin] [NBP1-82422] - Standard curve

Kit Contents for Human MMP-9 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MMP-9
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human MMP-9 polyclonal antibody
  • Human MMP-9 Standard lyophilized, 30 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-9

MMP-9 is a Zn+2 dependent endopeptidase, synthesized and secreted in monomeric form as zymogen. The structure is almost similar to MMP2, another member of matrixmetalloproteinase family. Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. Activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3. MMP-9 plays an important role in tumor invasion, angiogenesis and metastasis. Increased levels of MMP-9 have been demonstrated in colorectal cancer, in acute leukemia, breast cancer, human melanoma and bladder cancer. MMP-9 potentiates pulmonary metastasis formation and glioma invasion. Apart from its function in tumor formation and spread, MMP-9 with its destructive effects is in close association with lung diseases and asthma. Leukocyte recruitment to the central nervous system MMP-9 means an involvement in the pathogenesis of multiple sclerosis. MMP-9 has a crucial role in reproductive endocrinology and shows a changed expression pattern after myocardial infarction.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Human MMP-9 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human MMP-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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