Mouse CXCL1/KC Quantikine ELISA Kit

Mouse CXCL1/GRO alpha/KC/CINC-1 ELISA Standard Curve

Detection of Mouse CXCL1/GRO alpha/KC/CINC-1 by ELISA

STAT3 regulates chemoattractive CXCL1 expression.(a) ELISA of CXCL1 levels in lung lysates and BAL at 6 and 13 weeks post AdCre. Data were analysed by Student’s t-test and shown as mean±s.d., n≥7 mice per genotype and time point. (b) Representative in situ hybridization with probes specific for murine Cxcl1 (AS) in lung tumours at 13 weeks post AdCre. Sense probes (S) were used as negative control and surfactant protein C (SP-C) as lung epithelial maker. Scale bar, 100 μm. (c) Kaplan–Meier plot showing overall survival of lung adenocarcinoma patient samples harbouring smoking history with high or low IL-8 mRNA expression (expression values of upper quartile, log-rank test) n≥35–104 per group. (d) Human A549 lung cancer cell line (KRASG12S mutated) was stimulated with designated cytokines for indicated time points. IL-8 or CXCL1 expression was analysed via qRT–PCR. Data were analysed by one-way ANOVA with Tukey’s multiple comparison test and shown as mean±s.d. (e) A549 cells transducted with lentiviral vectors expressing nonspecific scrambled small hairpin (sh)RNA or shRNA against STAT3 were stimulated with indicated cytokines for 60 min. Data were analysed by one-way ANOVA with Tukey’s multiple comparison test and shown as mean±s.d. (f) Primary pneumocytes were isolated and infected with AdCre. 120 h post infection cells were stimulated with indicated cytokines for 60 min. Cxcl1 expression was analysed by qRT–PCR. Data are shown as mean±s.e.m. (g) Murine 3T3 fibroblasts and STAT3 overexpressing 3T3 fibroblasts were stimulated as in f. Cxcl1 expression was analysed by qRT–PCR. Data were analysed by one-way ANOVA with Tukey’s multiple comparison test and shown as mean± s.d. Values in d–g are presented as fold change of relative mRNA expression compared with each unstimulated individual cell line. At least two independent experiments with three individual plates per stimulation were performed. For all graphs: *P0.05; **P0.01; ***P0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms7285), licensed under a CC-BY license. Not internally tested by R&D Systems.