Mouse IL-12 p70 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M1270
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
2.5 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse IL-12 p70 Quantikine ELISA Kit
The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-12 p70
Cross-reactivity
0.5% cross-reactivity observed with available related molecules. 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 36 | 44 | 36 |
| Mean (pg/mL) | 28 | 68 | 193 | 20 | 56 | 230 |
| Standard Deviation | 2.5 | 6.5 | 19.4 | 2.3 | 6.2 | 20.0 |
| CV% | 8.9 | 9.6 | 10.1 | 11.5 | 11.1 | 8.7 |
Recovery for Mouse IL-12 p70 Quantikine ELISA Kit
The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 101 | 95-106 |
| Serum (n=7) | 97 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse IL-12 p70 Quantikine ELISA Kit
Mouse IL-12 p70 ELISA Standard Curve
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12 p70
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Long Name
Interleukin 12 p70
Alternate Names
IL12 p70
Gene Symbol
IL12A
Additional IL-12 p70 Products
Product Documents for Mouse IL-12 p70 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-12 p70 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.