Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M2728
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.7 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
The Quantikine Mouse IL-27 p28 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-27 p28 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse IL-27 p28 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-27 p28 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-27 p28.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-27 p28
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 31 | 33 | 37 |
| Mean (pg/mL) | 20.0 | 49.2 | 228 | 24.1 | 56.7 | 238 |
| Standard Deviation | 1.8 | 3.4 | 14.6 | 2.3 | 4.5 | 21.3 |
| CV% | 9.0 | 6.9 | 6.4 | 9.5 | 7.9 | 8.9 |
Recovery for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
The recovery of mouse IL-27 p28 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=8) | 102 | 83-112 |
| EDTA Plasma (n=8) | 99 | 83-116 |
| Heparin Plasma (n=8) | 95 | 87-101 |
| Serum (n=8) | 95 | 85-102 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse IL-27 p28 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
Mouse IL-27 p28/IL-30 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-27 p28/IL-30
Long Name
Interleukin 27 p28
Alternate Names
IL-30, IL27 p28
Gene Symbol
IL27
Additional IL-27 p28/IL-30 Products
Product Documents for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.