Mouse TIMP-1 Quantikine ELISA Kit Best Seller

Name: Mouse TIMP-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MTM100
Rating: 4 (3 reviews)

R&D Systems, part of Bio-Techne | Catalog # MTM100

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (17 µL), Serum (17 µL), EDTA Plasma (17 µL)

Sensitivity

3.5 pg/mL

Assay Range

37.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

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Product Summary for Mouse TIMP-1 Quantikine ELISA Kit

The Quantikine Mouse TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TIMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse TIMP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TIMP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse TIMP-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse

Specificity

Natural and recombinant mouse TIMP-1. This kit detects approximately 80% of the recombinant mouse TIMP-1 complexed with the pro-form of recombinant mouse MMP-9 and approximately 60% when complexed with active recombinant mouse MMP-9.

Cross-reactivity

0.5% cross-reactivity observed with available related molecules. 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	91.8	282	721	89.7	293	721
Standard Deviation	9.4	12.6	23.0	6.5	22.1	41.4
CV%	10.2	4.5	3.2	7.2	7.5	5.7

Recovery for Mouse TIMP-1 Quantikine ELISA Kit

The recovery of mouse TIMP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	102	92-112
EDTA Plasma (n=4)	107	92-119
Serum (n=6)	101	84-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse TIMP-1 in each matrix were diluted with Calibrator Diluent and assayed.

Scientific Data Images for Mouse TIMP-1 Quantikine ELISA Kit

Mouse TIMP-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol