DFHBI 1T

Name: DFHBI 1T
Brand: Tocris Bioscience
SKU: 5610

Tocris Bioscience, part of Bio-Techne | Catalog # 5610

Citations (19)

COA / SDS

GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers

Key Product Details

Description:	GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Chemical Name:	(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	320.21

View Full Technical Details for DFHBI 1T

Technical Data
Citations

Biological Activity for DFHBI 1T

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (K_d = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

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Technical Data for DFHBI 1T

M. Wt	320.21
Formula	C₁₃H₉F₅N₂O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1539318-36-9
PubChem ID	101889712
InChi Key	AWYCLBWNRONMQC-WMZJFQQLSA-N
Smiles	O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DFHBI 1T

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	32.02	100
	ethanol	16.01	50

Preparing Stock Solutions for DFHBI 1T

The following data is based on the product molecular weight 320.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.23 mL
5 mM	0.62 mL	3.12 mL	6.25 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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References for DFHBI 1T

References are publications that support the biological activity of the product.

Dey Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 2022 PMID: 34937909
Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
Filonov In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 2015 PMID: 26000751
Svensen and Jaffrey Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 2016 PMID: 26877022
Song Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 2014 PMID: 24393009