DMHBO+

Name: DMHBO+
Brand: Tocris Bioscience
SKU: 7764

Tocris Bioscience, part of Bio-Techne | Catalog # 7764

COA / SDS

Fluorescent upon binding to Chili aptamer; for imaging RNA in cells

Key Product Details

Description:	Fluorescent upon binding to Chili aptamer; for imaging RNA in cells
Chemical Name:	4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide
Purity:	≥98% (HPLC)
Molecular Weight:	552.37

View Full Technical Details for DMHBO+

Technical Data
Citations

Biological Activity for DMHBO+

DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.

Optical Data for DMHBO+

λ_abs	456 nm
λ_em	592 nm
Quantum Yield (φ)	0.1
Application	Time-resolved fluorescence energy transfer (TR-FRET)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for DMHBO+

M. Wt	552.37
Formula	C₂₂H₂₅IN₄O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2322286-81-5
InChi Key	KRIJKRIPLDZXHI-UHFFFAOYSA-N
Smiles	O/N=C/C(N(C1=CC=C(C=C1)[N+](C)(C)C)C/2=O)=NC2=C/C3=CC(OC)=C(C(OC)=C3)O.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DMHBO+

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	27.62	50

Preparing Stock Solutions for DMHBO+

The following data is based on the product molecular weight 552.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.62 mL	18.10 mL	36.21 mL
2.5 mM	0.72 mL	3.62 mL	7.24 mL
5 mM	0.36 mL	1.81 mL	3.62 mL
25 mM	0.07 mL	0.36 mL	0.72 mL

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Reconstitution Calculator

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References for DMHBO+

References are publications that support the biological activity of the product.

Steinmetzger A multicolor large stokes shift fluorogen-activating RNA aptamer with cationic chromophores. Chemistry 2019 PMID: 30485561