Flutax 1

Name: Flutax 1
Brand: Tocris Bioscience
SKU: 2226

Tocris Bioscience, part of Bio-Techne | Catalog # 2226

COA / SDS

Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy

Key Product Details

Description:	Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy
Chemical Name:	(2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity:	≥95% (HPLC)
Molecular Weight:	1283.3

View Full Technical Details for Flutax 1

Technical Data
Citations

Biological Activity for Flutax 1

Key information: Flutax 1 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.

Used for: direct imaging of the microtubule cytoskeleton.

Application: fluorescent microscopy.

Properties and Photophysical Data: Flutax 1 binds microtubules with high affinity (Ka ~ 10⁷M^-1). Absorption, fluorescence and fluorescence decay of Flutax 1 in solution are pH sensitive. Excitation and emission maxima (λ) are 495 nm and 520 nm, respectively.

Scientific Data Examples for Flutax 1

	Flutax 1 labeled HeLa cells. Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Flutax 1

M. Wt	1283.3
Formula	C₇₁H₆₆N₂O₂₁
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	191930-58-2
PubChem ID	16760423
InChi Key	JMWJJMCMRRQCSY-ADONSEPTSA-N
Smiles	O=C2[C@@]3(C)C([C@@]4(OC(C)=O)[C@H](OC4)C[C@@H]3OC([C@H](C)NC(C7=CC=C(C8=C(C=C%10)C(OC9=C8C=CC(O)=C9)=CC%10=O)C(C(O)=O)=C7)=O)=O)[C@H](OC(C6=CC=CC=C6)=O)[C@@]1(O)[C@](C)(C)C([C@H]2OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H]([C@]5=CC=CC=C5)NC(C%11=CC=CC=C%11)=O)=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Flutax 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	128.33	100
	ethanol	128.33	100

Preparing Stock Solutions for Flutax 1

The following data is based on the product molecular weight 1283.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.78 mL	3.90 mL	7.79 mL
5 mM	0.16 mL	0.78 mL	1.56 mL
10 mM	0.08 mL	0.39 mL	0.78 mL
50 mM	0.02 mL	0.08 mL	0.16 mL

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References for Flutax 1

References are publications that support the biological activity of the product.

Diaz Fast kinetics of Tax. binding to microtubules. J.Biol.Chem. 2003 PMID: 12496245
Evangelio Fluorescent taxoids as probes of the microtubule cytoskeleton. Cell Motil.Cytoskel. 1998 PMID: 9453715
Diaz Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 2000 PMID: 10818101