Laurdan
Tocris Bioscience, part of Bio-Techne | Catalog # 7275
Key Product Details
| Description: | Polarity sensitive lipid membrane fluorescent probe. Used for: imaging lipid rafts. Application: fluorescent microscopy |
| Chemical Name: | 1-[6-(Dimethylamino)-2-naphthalenyl]-1-dodecanone |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 353.55 |
Biological Activity for Laurdan
Key information: Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). Suitable for live and fixed cells as well as in whole tissues imaging with multiphoton microscopy.
Used for: imaging lipid raft and cell membrane investigations.
Application: fluorescence microscope. Suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS).
Properties and Photophysical Data: When Laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids. Excitation and emission maxima (λ) are 366 nm and 497 nm, respectively; quantum yield = 0.61; extinction coefficient = 19,500 M-1cm-1. In gel and liquid phase membranes, excitation and emission maxima (λ) are 440 nm and 490 nm, respectively.
Optical Data for Laurdan
| Emission Color | Blue |
|---|---|
| λabs | 366 nm |
| λem | 497 nm |
| Extinction Coefficient (ε) | 19500 M-1cm-1 |
| Quantum Yield (φ) | 0.61 |
| Application | Polarity Sensitive Fluorescent Probe for Lipid Raft and Cell Membrane Investigations |
| Alternative For | C-Laurdan, Di-4-ANEPPDHQ |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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