NBD-PE

Name: NBD-PE
Brand: Tocris Bioscience
SKU: 7538

Tocris Bioscience, part of Bio-Techne | Catalog # 7538

COA / SDS

Green-fluorescent label for lysosomal lipid bodies. Used for: Application: confocal microscopy

Key Product Details

Description:	Green-fluorescent label for lysosomal lipid bodies. Used for: Application: confocal microscopy
Chemical Name:	Triethylammonium (R)-2,3-bis(palmitoyloxy)propyl(2-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)ethyl)phosphate
Purity:	≥95% (HPLC)
Molecular Weight:	956.26

View Full Technical Details for NBD-PE

Technical Data
Citations

Biological Activity for NBD-PE

Key information: NBD-PE is a fluorescent label for lysosomal lipid bodies.

Used for: quantitatively measure phospholipidosis, which is useful to screen drugs for SARS-CoV-2 repurposing. NBD-PE can also be used to label giant unilamellar vesicles (GUV).

Application: confocal microscopy, fluorescence recovery after photobleaching (FRAP).

Properties and Photophysical Data: It consists of a single tail (16:0) phospholipid derivative with an NBD fluorophore conjugated to the head group. Excitation and emission maxima (λ) are 463 nm and 536 nm, respectively, extinction coefficient = 22,000 M^-1cm^-1.

Optical Data for NBD-PE

Emission Color	Green
λ_abs	463 nm
λ_em	536 nm
Extinction Coefficient (ε)	22000 M^-1cm^-1
Application	Confocal Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for NBD-PE

M. Wt	956.26
Formula	C₄₃H₇₅N₄O₁₁P.C₆H₁₅N
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	178119-00-1
InChi Key	LEJHDNAGUHKMLR-GKEJWYBXSA-N
Smiles	CC[N+](CC)([H])CC.CCCCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)COP([O-])(OCCNC1=CC=C(N(=O)=O)C2=NON=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NBD-PE

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	methanol	0.48	0.5 with sonication

Preparing Stock Solutions for NBD-PE

The following data is based on the product molecular weight 956.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.005 mM	209.15 mL	1,045.74 mL	2,091.48 mL
0.025 mM	41.83 mL	209.15 mL	418.30 mL
0.05 mM	20.91 mL	104.57 mL	209.15 mL
0.25 mM	4.18 mL	20.91 mL	41.83 mL

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Reconstitution Calculator

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References for NBD-PE

References are publications that support the biological activity of the product.

Tummino Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2. Science 2021 PMID: 34326236
Gum Analysis of two matrix metalloproteinase inhibitors and their metabolites for induction of phospholipidosis in rat and human hepatocytes. Biochem.Pharmacol. 2001 PMID: 11755120
Li Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays. Nat.Commun. 2020 PMID: 31932592
Morelli Validation of an in vitroscreen for phospholipidosis using a high-content biology platform. Cell Biol.Toxicol. 2006 PMID: 16463016