Read to see how MauriceFlex fractionation enables intact and peptide mapping analysis by LC-MS using a NIST mAb sample. You'll learn how:
|
I need to collect charge variant fractions, but IEX workflows are complicated and time consumingMauriceFlex saves you weeks, even months, of time spent on method development and bridging studies by giving you charge analysis and charge variant fractionation on the same instrument. Finish analysis and collect fractions all in the same day. |
|
How do I know my mass spec platform is compatible with MauriceFlex?MauriceFlex gives you the ultimate flexibility, because you can take your collected fractions and not only analyze them on any mass spec platform, but also perform other assays such as peptide mapping. |
|
My samples have urea and I need to collect fractions for mass spec analysisMauriceFlex lets you collect charge variant fractions of your samples, even if they contain urea or methylcellulose. Other capillary electrophoresis-mass spec coupled systems aren’t compatible with samples containing urea. |
View all citations for the Imaged Capillary Electrophoresis (iCE) platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
"Replacing preparative chromatography with MauriceFlex has made the process of fraction collection more efficient."
Amreen Jonas, Principal Associate Scientist, Mersana Therapeutics
"I think that the data we produced [on Maurice] shows excellent quality, due to the precise and reliable Maurice instrument."
Professor Hermann Wätzig, Technical University of Braunschweig in Germany
"The short run times and easy sample preparation allowed for method development within a day or two. The versatility of the instrument allows the possibility of offering clients rapid alternatives to traditional ID and charge heterogeneity assays."
Joan Garrison, QC Method Transfer Scientist, Cook Pharmica
| Feature | cIEF | CE-SDS PLUS | Turbo CE-SDS | cIEF Fractionation |
|---|---|---|---|---|
| Minimum Sample Volume | 50 µL | 50 µL | 100 µL | 100 µL |
| Sample Delivery | Vacuum | Electrokinetic | Electrokinetic | Vacuum |
| Typical Separation Time | 6–10 min (molecule-dependent) | Reduced IgG: 25 min, Non-reduced IgG: 35 min | Reduced IgG: 5.5 min Non-reduced IgG: 8 min | 40 – 50 min (molecule-dependent) |
| Detection Capability |
UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320– 450 nm |
UV Absorbance at 220 nm | UV Absorbance at 220 nm | Fluorescence: Ex 280 nm, Em 320-450nm |
| Typical Voltage | Pre-focusing: 1500 V, focusing: 3000 V | Separation: 5750 V | Separation: 4200 V |
Pre-focusing: 500 V and 1000 V Focusing: 1500 V |
| Sample Injections per Cartridge | 100 guaranteed, 200 maximum (max 25 batches) | 100 guaranteed, 500 maximum (max 25 batches) | 100 guaranteed (max 25 batches) | Maximum 15 injections |
| Maximum Sample Injections per Batch | 100 | 48 | 96 |
1 (fractionation) 4 (cIEF) |
| pI/Size Range | 2.85–10.45 | 10–270 kDa | 10–270 kDa | 3-10 |
| pI/Sizing CV | 1% | ≤2% | <2% | 1% |
| CV for Peaks >10% Composition | ≤5% (Intra-batch), ≤6% (Inter-batch) | N/A | N/A | ≤10% (Inter-batch) |
| Relative Migration Time CV | N/A | <1% for reduced IgG | <5% | N/A |
| pI/Sizing Resolution | 0.05 pI units (for wide range 3–10 ampholytes) | ≥1.5 for NGHC/HC IgG Standard | ≥1.0 for NGHC/HC IgG Standard | N/A |
| Dynamic Range | 2 logs | 2 logs | 2 logs | N/A |
| Linearity | >0.995 | >0.995 | >0.995 | N/A |
| Sensitivity (LOD) |
0.7 µg/mL (Native fluorescence) 3.0 µg / mL(Absorbance) (Values based on a monoclonal antibody) |
0.3 µg/mL (Value based on Internal Standard) | 0.6 µg/mL (Value based on Internal Standard) | N/A |
| Sample Tray Options | 96-well plates or 48 vials | 96-well plates only | ||
| Power | 100 V–240 V (AC), 50/60 Hz, 500 W | |||
| Voltage Range | 0–6,500 V | |||
| Temperature Control Range | 4-25 °C | 10-25 °C | ||
| Dimensions | 44cm H x 42cm W x 61cm D | |||
| Weight | 46 kg (100 lb) | |||
