Recombinant Human MICA His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34993
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 320 amino acids corresponding to MICA with a His tagSource: E. coli
Amino Acid Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHKLGCFGG
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of MICA as determined by LAL method.
Predicted Molecular Mass
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
MICA Protein is fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-34993
| Formulation | 0.2 um filtered concentrated sterile solution in PBS, pH 7.4, and 8 M Urea. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile dH2O to a conc. of 1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Make further dilutions in appropriate buffered solutions, with 8M Urea. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: MICA
Long Name
MHC Class I-related Protein A
Alternate Names
FLJ60820, MGC111087, PERB11.1
Gene Symbol
MICA
Additional MICA Products
Product Documents for Recombinant Human MICA His Protein
Product Specific Notices for Recombinant Human MICA His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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