Recombinant Human PDGF-AA Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07102

Novus Biologicals, part of Bio-Techne
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NBP3-07102-100ug
NBP3-07102-10ug
NBP3-07102-1mg
NBP3-07102-500ug
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all PDGF-AA Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 126 amino acid polypeptide chains corresponding to PDGF-AA. However it migrates with an apparent molecular mass of 336 kDa in SDS-PAGE Source: E. coli

Amino Acid Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 0.1 EU/ug of PDGF-AA as determined by LAL method.

Predicted Molecular Mass

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Biological activity measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for tis effect is 50-200 ng/mL in a fluorometric assay using a redox sensitive dye.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP3-07102
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name

Platelet-derived Growth Factor AA

Alternate Names

PDGFAA

Gene Symbol

PDGFA

Additional PDGF-AA Products

Product Documents for Recombinant Human PDGF-AA Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PDGF-AA Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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