Recombinant Human BMP-4 Protein
R&D Systems, part of Bio-Techne | Catalog # 314-BP
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293-Arg408
Ser293-Arg408
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser293
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions
35-41 kDa, non-reducing conditions
Activity
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect
is 0.70-7.00 ng/mL.
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is 3.00-30.0 ng/mL.
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is 3.00-30.0 ng/mL.
Reviewed Applications
Read 13 reviews rated 4.9 using 314-BP in the following applications:
Scientific Data Images for Recombinant Human BMP-4 Protein
Recombinant Human BMP-4 Protein Bioactivity
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL.
Recombinant Human BMP-4 Protein SDS-PAGE
1 μg/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19-24 kDa and 35-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 314-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 314-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 50-200 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMP-4
BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).
References
- Zhang, P. et al. (2008) Blood 111:1933.
- Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
- Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
- Sadlon, T.J. et al. (2004) Stem Cells 22:457.
- Frank, D.B. et al. (2005) Circ. Res. 97:496.
- Wozney, J. et al. (1988) Science 242:1528.
- Cui, Y. et al. (1998) EMBO J. 17:4735.
- Cui, Y. et al. (2001) Genes Dev. 15:2797.
- Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
- Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
- Chen, D. et al. (2004) Growth Factors 22:233.
- Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
- Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
- Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
- Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
UniProt
Additional BMP-4 Products
Product Documents for Recombinant Human BMP-4 Protein
Product Specific Notices for Recombinant Human BMP-4 Protein
For research use only
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