Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10703

R&D Systems, part of Bio-Techne
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AVI10703-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P10721.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD117/c-kit Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD117/c-kit protein
Human CD117/c-kit
(Gln26-Thr520)
Accession # P10721.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26 (Blocked)

Predicted Molecular Mass

84 kDa

SDS-PAGE

100-120 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SCF (Mammalian-expressed, Catalog # 7644-SC) is coated at 1 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag binds with an ED50 of 0.05-0.30   μg/mL.

Scientific Data Images for Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag Binding Activity.

When Recombinant Human SCF (Mammalian-expressed) Protein (7466-SC) is coated at 1 ug/mL, 100 uL/well, Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag binds with an ED50 of 0.05-0.30 μg/mL.

Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein SDS PAGE.

2 μg/lane of Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag (Catalog # AVI10703) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Formulation, Preparation and Storage

AVI10703
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (SCF R), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF (1, 2). Mature human SCF R consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCF R shares 73% and 76% amino acid sequence identity with mouse and rat SCF R, respectively. At least 4 SCF R isoforms, produced by alternative mRNA splicing, have been identified in humans and 2 are characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (3, 4). Human SCF R is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1, 2, 5, 6). SCF R interacts with noncovalent dimers of transmembrane or soluble SCF to trigger receptor dimerization and initiate signaling (7). Our Avi-tag Biotinylated rhSCF R features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
  2. Ashman, L.K. et al. (1999) Inter Jour of Biochem & Cell Bio. 31:1037.
  3. Lennartsson J. and Rönnstrand L. (2012) Physiol Rev. 92:1619
  4. Caruana, G. et al. (1999) Oncogene 18:5573
  5. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  6. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
  7. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P10721.1

Additional CD117/c-kit Products

Product Documents for Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

For research use only

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