Recombinant Human GDNF Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 212-GD
Analyzed by SEC-MALS
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity, Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human GDNF protein
Arg109-Ile211
Arg109-Ile211
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg109
Predicted Molecular Mass
11.6 kDa (monomer)
Activity
Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells.
The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714‑GR).
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714‑GR).
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
Reviewed Applications
Read 4 reviews rated 4.8 using 212-GD in the following applications:
Scientific Data Images for Recombinant Human GDNF Protein
Recombinant Human GDNF Protein SEC-MALS.
Recombinant human GDNF (Catalog # 212-GD) has a molecular weight (MW) of 27.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human GDNF Protein Bioactivity
Recombinant Human GDNF (Catalog # 212-GD) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFRa-1 Fc Chimera (Catalog # 714-GR). The activity is approximately 9-fold greater than the top competitor's GDNF.
Recombinant Human GDNF Protein SDS-PAGE
1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 212-GD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 212-GD/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GDNF
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Long Name
Glial Cell line-derived Growth Factor
Alternate Names
ATF, HFB1-GDNF, HGDNF, HSCR3
Gene Symbol
GDNF
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF Protein
Product Specific Notices for Recombinant Human GDNF Protein
For research use only
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