Recombinant Human TGF-beta 3 (CHO-expressed) Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 8420-B3
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 3 protein
Ala301-Ser412
Ala301-Ser412
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala301
Predicted Molecular Mass
13 kDa
SDS-PAGE
9-11 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.01-0.04 ng/mL.
The ED50 for this effect is 0.01-0.04 ng/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 8420-B3 in the following applications:
Scientific Data Images for Recombinant Human TGF-beta 3 (CHO-expressed) Protein
Recombinant Human TGF-beta 3 (CHO-expressed) Protein Bioactivity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.01-0.04 ng/mL.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 8420-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 8420-B3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 50 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TGF-beta 3
TGF-beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and beta2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF- beta3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-like convertase to generate a 220 aa latency-associated peptide (LAP) and a 112 aa mature TGF-beta 3 (10, 11). Mature human TGF- beta3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF- beta3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases (12, 13). It can also be activated by extreme pH and reactive oxygen species (1-5, 12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF- beta RI or ALK-1, to modulate transcription through Smad phosphorylation (14-16). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF- beta RIII and endoglin) and the subsequent initiation of Smad-dependent or -independent signaling pathways (15, 17, 18).
References
- Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
- Doetschman, T. et al. (2012) Genesis 50:59.
- Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
- Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
- Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
- Chang, H. et al. (2002) Endocr. Rev. 23:787.
- Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
- Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
- Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
- Derynck, R. et al. (1988) EMBO J. 7:3737.
- Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
- Munger, J.S. et al. (1997) Kidney Int 51:1376.
- Wipff, P.J. and B. Hinz (2008) Eur J Cell Biol 87:601.
- Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
- de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
- Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
- Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
- Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.
Long Name
Transforming Growth Factor beta 3
Alternate Names
ARVD1, LDS5, RNHF, TGFB3, TGFbeta 3
Gene Symbol
TGFB3
UniProt
Additional TGF-beta 3 Products
Product Documents for Recombinant Human TGF-beta 3 (CHO-expressed) Protein
Product Specific Notices for Recombinant Human TGF-beta 3 (CHO-expressed) Protein
For research use only
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