Recombinant Mouse SECTM1A Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7837-ST

R&D Systems, part of Bio-Techne
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7837-ST-050
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Key Product Details

Source

NS0

Accession #

NP_663348

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all SECTM1A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SECTM1A protein
Mouse SECTM1A
(Gln28-Thr165)
Accession # NP_663348		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln28 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

42.6 kDa (monomer)

SDS-PAGE

55-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse SECTM1A Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.

Scientific Data Images for Recombinant Mouse SECTM1A Fc Chimera Protein, CF

Recombinant Mouse SECTM1A Fc Chimera Protein Binding Activity

Recombinant Mouse SECTM1A Fc Chimera Protein Binding Activity

When Recombinant Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 µg/mL, Recombinant Mouse SECTM1A Fc Chimera (Catalog # 7837-ST) binds with an ED50 of 30-150 ng/mL.

Formulation, Preparation and Storage

7837-ST
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SECTM1A

SECTM1A (secreted and transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).

References

  1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
  2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
  3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
  4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
  5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
  6. Swiss-Prot Accession # Q921W8.

Long Name

Secreted and Transmembrane 1A

Alternate Names

K12, SECTM

Entrez Gene IDs

209588 (Mouse); 287885 (Rat)

Gene Symbol

SECTM1A

UniProt

NP_663348

Additional SECTM1A Products

Product Documents for Recombinant Mouse SECTM1A Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse SECTM1A Fc Chimera Protein, CF

For research use only

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