Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0113
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Summary
for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody
Immunogen
Rat IgG heavy and light chains
Specificity
Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
Detection of Rat IgG Primary Antibody by Flow Cytometry
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994, filled histogram) or isotype control antibody (Catalog # MAB006), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) at 10 µL/106cells.Applications for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where
the primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommended
concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
Product Specific Notices for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
For research use only
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