Llama IgG Antibody
R&D Systems, part of Bio-Techne | Catalog # AF011
Key Product Details
Species Reactivity
Llama
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Llama IgG Antibody
Immunogen
Lllama IgG
Specificity
Detects Llama IgG in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Llama IgG Antibody
Detection of Siglec-2/CD22 in HEK293 Human Cell Line Transfected with Human Siglec-2/CD22 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-2/CD22 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-2/CD22 Llamabody VHH Monoclonal Antibody (LMAB10732) followed by Goat Anti-Llama Secondary Antibody (Catalog # AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins.Applications for Llama IgG Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Cells stained with primary llama IgG antibody, followed with fluorescently lableled tertiary anti-goat IgG antibody
Sample: Cells stained with primary llama IgG antibody, followed with fluorescently lableled tertiary anti-goat IgG antibody
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Llama IgG Antibody
Product Specific Notices for Llama IgG Antibody
For research use only
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