Do the AAV assays recognize fully-assembled capsids?

Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.

Do the AAV assays recognize both natural and recombinant AAV?

Yes, the AAV assays detect both natural and recombinant AAV.

Does the AAV1 assay recognize other AAV serotypes?

This assay recognizes intact AAV1 capsids. The AAV1 antibody (ADK1a) used in this assay cross-reacts with AAV6 and AAV12.

Do the Simple Plex AAV assays distinguish between full and empty capsids?

No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.

What diluent is provided with the AAV assays?

The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.

What is the minimum required dilution (MRD) for bio-process samples?

All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.

Simple Plex AAV1 Cartridge

Name: Simple Plex AAV1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-007969

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007969

Product Datasheet / COA

Simple Plex AAV1 assay kit contains cartridge, sample diluent SD19, and wash buffer. Part # starting with ST01 ships in 2-3 days.

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Simple Plex AAV1 Cartridge Summary:

Simple Plex assay for the detection of Adeno-Associated Virus type 1 (AAV1) intact capsids in bioprocess samples. This assay uses PROGEN's AAV1 (ADK1a) antibody.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex AAV1 Cartridge

Technical Data
Citations / FAQs

Product Specifications for Simple Plex AAV1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Linearity

Samples containing and/or spiked with high concentrations of AAV1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex AAV1 Cartridge

	AAV1 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: A4GNT

Adeno-Associated Virus Subtype 1 (AAV1) is a vector often used in gene delivery systems.

Item	Value
Entrez Gene IDs	51146 (Human); 333424 (Mouse); 685758 (Rat)
Alternate Names	A4GNT, alpha-1,4-N-acetylglucosaminyltransferase, alpha4GnT

FAQs for Simple Plex AAV1 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex AAV1 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol