Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Name: Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002574

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002574

Simple Plex Cartridge Kit containing IFN-g for use with Human Plasma/Serum. Includes wash buffer and sample diluent SD13. For use with the Ella automated immunoassay system.

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Key Product Details

Sample Type & Volume Required Per Well:	Cell Culture Supernates (5 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.17 pg/mL
Assay Range:	0.17 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human IFN-gamma (3rd Gen) Cartridge Summary:	Simple Plex assay for the detection of human IL-6 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Reactivity	Human
Specificity	Natural and recombinant human IFN-gamma.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	21.1	1175	23.4	1192
Standard Deviation	1.48	33.4	2.52	99.4
CV%	7	2.8	10.8	8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=6)	94	91-98
EDTA Plasma (n=4)	89	88-90
Heparin Plasma (n=4)	90	83-99
Serum (n=4)	95	83-102

Linearity

Samples containing and/or spiked with high concentra- tions of human IFN-gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

	Human IFN-gamma Simple Plex Cartridge Kit for use with the Ella Automated Immunoassay System
	IFN-gamma Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.